![]() ![]() ![]() The present study aimed to evaluate the antibacterial activity of extracts of A. Bacterial cellulose membrane (nature BCM) is a potential carrier as a drug delivery system in the wound and burn treatment. Previous studies about anti-inflammatory, antimicrobial and antioxidant properties of Aloe vera (L.) Burm., Calendula officinalis L. are acknowledge by antimicrobial effects. Previous studies about anti-inflammatory, antimicrobial and antioxidant properties of Aloe vera (L.) Burm., Calendula officinalis L.and Matricaria recutita L. Tel: 55+ (15) 98153-6692 Email: for Correspondence: Luciane Lopes, Seriema - Evidence Service for Monitoring and Evaluation, University of Sorocaba, Sorocaba, Brazil, Tel: +55 (15) 2101-7104 Email: injuries induce a state of immunodepression that predisposes to a bacterial infectious complication that leads to several comorbid diseases and high mortality rate. *Address for Correspondence: Marco Chaud, Laboratory of Biomaterial and Nanotechnology (LaBNUS), University of Sorocaba, Brazil, University of Sorocaba, BrazilĤLaboratory of Industrial Microbiology and Fermentation Process, University of Sorocaba, BrazilĥSeriema - Evidence Service for Monitoring and Evaluation, University of Sorocaba, Sorocaba, Brazil University of Sorocaba, BrazilĢPPGCF – Post-Graduate Program in Pharmaceutical Sciences of the University of Sorocaba, Sorocaba/SP, BrazilģCollege of Pharmacy. In light of these alterations, the potential drawbacks of using B6J- Nnt MUT mice in biomedical research should not be overlooked.Medicinal plant extract associated with bacterial cellulose membrane: Antibacterial activity and physicochemical propertiesįernando Batain 1, Kessi Crescencio 1, Thais Alves 1, Juliana Ferreira Souza 1, Venâncio Amaral 1, Juliana Castro 2, Carolina Santos 3, Angela Jozala 4, Luciane Lopes 5* and Marco Chaud 1*ġLaboratory of Biomaterial and Nanotechnology (LaBNUS). Altogether, our data suggest that NNT functions as a high-capacity source of mitochondrial NADPH and that its functional loss due to the Nnt mutation results in mitochondrial redox abnormalities, most notably a poor ability to sustain NADP and glutathione in their reduced states. Nonetheless, the maximal activity of NADP-dependent isocitrate dehydrogenase, which is a coexisting source of mitochondrial NADPH, was similar between both groups. ![]() In addition, the mitochondria of B6J- Nnt MUT mice exhibited increased oxidized/reduced glutathione ratios as compared to B6JUnib- Nnt W mice. The functional evaluation of respiring mitochondria revealed major redox alterations in B6J- Nnt MUT mice, including an absence of transhydrogenation between NAD and NADP, higher rates of H 2O 2 release, the spontaneous oxidation of NADPH, the poor ability to metabolize organic peroxide, and a higher susceptibility to undergo Ca 2+-induced mitochondrial permeability transition. Liver mitochondria were isolated both from an Nnt wild-type C57BL/6 substrain (B6JUnib- Nnt W) and from B6J- Nnt MUT mice. Here, we characterize the consequences of the Nnt mutation on the mitochondrial redox functions of B6J- Nnt MUT mice. A spontaneous Nnt mutation in C57BL/6J (B6J- Nnt MUT) mice arose nearly 3 decades ago but was only discovered in 2005. This enzyme catalyzes the reduction of NADP + at the expense of NADH oxidation and H + reentry to the mitochondrial matrix. Nicotinamide nucleotide transhydrogenase (NNT), an integral protein located in the inner mitochondrial membrane, contributes to an elevated mitochondrial NADPH/NADP + ratio. NADPH is the reducing agent for mitochondrial H 2O 2 detoxification systems. ![]()
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